Recombinant Human BTN3A3 Protein (rh BTN3A3) Gln 30 - Trp 248 (Accession # AAH15815) was produced in human 293 cells (HEK293) at ACROBiosystems.
rh BTN3A3 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 25.5 kDa. The predicted N-terminus is Gln 30. DTT-reduced Protein migrates as 28 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg of the rh BTN3A3 by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Butyrophilin subfamily 3 member A3 (BTN3A3),a member of the immunoglobulin superfamily and BTN/MOG family, is also known as BTF3, which contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A3 is detected in peripheral blood mononuclear cells ,T-cells ,spleen and lymphocytes. BTN3A3 also plays a role in T-cell responses in the adaptive immune response. Stimulation of human butyrophilin 3(BTN3) molecules results in negative regulation of cellular immunity.
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