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Your Position: Home > Protein > FcRn (FCGRT & B2M) > FCM-H8286

Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, ultra sensitivity (primary amine labeling) (HPLC & SPR verified)

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  • Synonym
    FcRn,FCGRT & B2M
  • Source
    Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling (FCM-H8286) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1 (FCGRT) & P61769-1 (B2M)). It is the biotinylated form of Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) (FCM-H5286).
    Predicted N-terminus: Ala 24(FCGRT) & Ile 21(B2M)
  • Molecular Characterization
    FcRn (FCGRT & B2M) Structure

    Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.2 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 33 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
FcRn (FCGRT & B2M) SDS-PAGE

Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
FcRn (FCGRT & B2M) SEC-HPLC

The purity of Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling (Cat. No. FCM-H8286) was greater than 90% as determined by SEC-HPLC.

Bioactivity-SPR
 FcRn (FCGRT & B2M) SPR

Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling (Cat. No. FCM-H8286) captured on Biotin CAP - Series S sensor Chip can bind Herceptin® with an affinity constant of 0.57 μM as determined in a SPR assay (Biacore 8K) (QC tested).

Bioactivity-BLI
 FcRn (FCGRT & B2M) BLI

Loaded Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling (Cat. No. FCM-H8286) on SA Biosensor, can bind Herceptin with an affinity constant of 0.36 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 FcRn (FCGRT & B2M) BLI

Loaded Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling (Cat. No. FCM-H8286) on SA Biosensor, can bind Human Serum Albumin, His Tag (Cat. No. HSA-H5220) with an affinity constant of 0.263 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
  • Clinical and Translational Updates

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  • Latest Research Phase:Approved

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