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Human SMAC / Diablo Protein  pdf  pdf  pdf


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Synonym

DIABLO,SMAC

Source

Recombinant Human DIABLO /SMAC Protein (rh DIABLO / SMAC) Ala 56 - Asp 239 (Accession # NP_063940) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

rh DIABLO / SMAC is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 21.7 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 22 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the rh DIABLO / SMAC by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 20 mM Hepes, 150 mM Nacl, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human DIABLO /SMAC  Protein
The purity of rh DIABLO / SMAC was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Diablo homolog, mitochondrial (DIABLO) is also known as direct IAP-binding protein with low pI and second mitochondria-derived activator of caspase (SMAC), which can interact with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Acting by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP),DIABLO can also promote apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Also, DIABLO inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Furthermore, DIABLO is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

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References

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