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Biotinylated Human B7-H4, ultra sensitivity (primary amine labeling)  pdf  pdf  pdf


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Synonym

B7-H4,VTCN1,B7S1,B7h.5

Source

MABSol® Biotinylated Human B7-H4 (B74-H8222) is expressed from human HEK293 cells. It contains AA Phe 29 - Ala 258 (Accession # NP_078902). It is the biotinylated form of Human B7-H4 protein, His Tag (Cat # B74-H5222).

Predicted N-terminus: Phe 29

Molecular Characterization

B7-H4(Phe 29 - Ala 258)NP_078902
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.1 kDa. The protein migrates as 43-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio

The biotin to protein ratio is 0.7-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Human B7-H4 (Catalog # B74-H8222) SDS-PAGE gel

Biotinylated Human B7-H4 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Biotinylated Human B7-H4 (Catalog # B74-H8222) ELISA bioactivity

Immobilized anti-B7-H4 mAb (Human IgG1) at 2 μg/mL can bind Biotinylated Human B7-H4 (Catalog # B74-H8222) with a linear range of 4-62 ng/mL.

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Background

V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.