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Human Neuregulin-4 / NRG4 Protein, HEK293 Expressed  pdf  pdf  pdf


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NR4-H52H3-50ug
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Synonym

Neuregulin 4,NRG4

Source

Human Neuregulin-4 / NRG4 Protein, HEK293 Expressed (Human NRG4) Pro 2 - Phe 62 (Accession # Q8WWG1) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Human NRG4 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 8.6 kDa. The predicted N-terminus is Pro 2. DTT-reduced Protein migrates as 13 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the Human NRG4 by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Human Neuregulin-4 / NRG4 Protein, HEK293 Expressed
The purity of Human NRG4 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Neuregulin 4 also known as NRG4 is a member of the neuregulin protein family which in humans is encoded by the NRG4 gene. Loss of expression of NRG4 is frequently seen in advanced bladder cancer while increased NRG4 expression correlates to better survival. The neuregulins, including NRG4, activate type-1 growth factor receptors (EGFR) to initiating cell-to-cell signaling through tyrosine phosphorylation. Furthermore, NRG4 is a low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. NRG4 does not bind to the ERBB1, ERBB2 and ERBB3 receptors.

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References

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