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Your Position: Home > Protein > IgG2a Fc > IGA-M8210

Biotinylated Mouse IgG2a Fc Protein, Avitag™ (MALS verified)

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  • Synonym
    IgG2A
  • Source
    Biotinylated Mouse IgG2a Fc, Avitag(IGA-M8210) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Lys 330 (Accession # P01863).
    Predicted N-terminus: Glu 98
  • Molecular Characterization
    IgG2a Fc Structure

    This protein carries an Avi tag (Avitag™) at the C-terminus.

    The protein has a calculated MW of 28.2 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IgG2a Fc SDS-PAGE

Biotinylated Mouse IgG2a Fc, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IgG2a Fc MALS images

The purity of Biotinylated Mouse IgG2a Fc, Avitag (Cat. No. IGA-M8210) is more than 90% and the molecular weight of this protein is around 52-70 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IgG2a Fc ELISA

Immobilized Recombinant Protein A at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG2a Fc, Avitag (Cat. No. IGA-M8210) with a linear range of 0.8-13 ng/mL (QC tested).

 IgG2a Fc BATCH ELISA
 IgG2a Fc ELISA

Immobilized Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG2a Fc, Avitag (Cat. No. IGA-M8210) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

  • Background
    Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
  • Clinical and Translational Updates

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