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Mouse CD40 Ligand / TNFSF5 Protein  pdf  pdf  pdf


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CDL-M5248-100ug
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Synonym

CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39

Source

Mouse CD40 Ligand / TNFSF5 Protein (Mouse CD40 Ligand, His Tag) Gly 115 - Leu 260 (Accession # P27548) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

polyhistidine

CD40 Ligand (Gly 115-Leu 260)P27548

Mouse CD40 Ligand, His Tag is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 16.8 kDa. The predicted N-terminus is His. The reducing (R) protein migrates as 21-25 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE

Mouse CD40 Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Immobilized Mouse CD40, Fc Tag (Catalog # TN5-M5259) at 2 μg/mL ( 100 μl/well ) can bind Mouse CD40 Ligand, His Tag (Catalog # CDL-M5248) with a linear range of 5-20 ng/mL.

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Background

CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.

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References