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Biotinylated Human CX3CL1, ultra sensitivity (primary amine labeling)  pdf  pdf  pdf


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Synonym

CX3CL1, Fractalkine

Source

MABSol® Biotinylated Human CX3CL1 (CX1-H8221) is expressed from human HEK293 cells. It contains AA Gln 25 - Gln 341 (Accession # AAH01163). It is the biotinylated form of Human CX3CL1 protein, His Tag (Cat. No. CX1-H5221).

Molecular Characterization

CX3CL1 (Gln 25-Gln 341)AAH01163

polyhistidine

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.5 kDa. The protein migrates as 50-90 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Biotinylation

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE


Biotinylated Human CX3CL1, ultra sensitivity (primary amine labeling)
Biotinylated Human CX3CL1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
 
 

Background

Fractalkine is also known as C-X3-C motif chemokine 1 (CX3CL1) and neurotactin. Fractalkine is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. Fractalkine is found commonly throughout the brain, particularly in neural cells, and its receptor is known to be present on microglial cells. It has also been found to be essential for microglial cell migration. CX3CL1 is also up-regulated in the hippocampus during a brief temporal window following spatial learning, the purpose of which may be to regulate glutamate-mediated neurotransmission tone. This indicates a possible role for the chemokine in the protective plasticity process of synaptic scaling.

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