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Recombinant Protein G  pdf  pdf  pdf


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RPG-S3140-2mg
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RPG-S3140-10mg
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Synonym

RPG, Protein G, ProteinG

Source

Recombinant Protein G Protein, also known as Recombinant Staphylococcus Aureus protein G148, was expressed in E.Coli at ACRObiosystems.

Molecular Characterization

Recombinant Protein G Protein was fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. DTT-reduced protein migrates as a 31 kDa polypeptide. Protein G Protein can bind to human IgG1 Fc .

Endotoxin

Less than 1.0 EU per μg of the protein by LAL method.

Purity

>98% as determined by SDS-PAGE of reduced Recombinant Protein G Protein.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

Contact us for customized product format or formulation.

Reconstitution

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).

 

SDS-PAGE


Protein G
The purity of Recombinant Protein G Protein was determined by SDS-PAGE of reduced Recombinant Protein G Protein and staining overnight with Coomassie Blue.
 

Background

Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) [1] cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from this recombinant form of Protein G.

antibody-structure

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.