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Mouse BAFFR / TNFRSF13C Protein, Fc Tag  pdf  pdf  pdf


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BAR-M5259-100ug
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BAR-M5259-1mg
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Synonym

BAFFR,TNFRSF13C,BAFF-R,BROMIX,CD268,CVID4,prolixin

Source

Mouse BAFFR / TNFRSF13C, Fc Tag (BAR-M5259) is expressed from human 293 cells (HEK293). It contains AA Ser 10 - Ala 71 (Accession # NP_082351.1).

Predicted N-terminus: Ser 10

Molecular Characterization

BAFFR(Ser 10 - Ala 71)NP_082351.1
Fc(Pro 100 - Lys 330)P01857

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 33.3 kDa. As a result of glycosylation, the protein migrates as 40-45 kDa under reducing (R) condition, and 80-100 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris,100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Mouse BAFFR / TNFRSF13C, Fc Tag (Catalog # BAR-M5259) SDS-PAGE gel

Mouse BAFFR / TNFRSF13C, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Mouse BAFFR / TNFRSF13C, Fc Tag (Catalog # BAR-M5259) ELISA bioactivity

Immobilized Human BAFF, His Tag (Catalog # BAF-H5248) at 5 μg/mL (100 μL/well) can bind Mouse BAFFR, Fc Tag (Catalog # BAR-M5259) with a linear range of 0.3-13 ng/mL.

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Background

BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), is a membrane protein of the TNF receptor superfamily which recognizes BAFF. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of BAFF in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.