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Mouse Galectin-4 / LGALS4 / L36LBP Protein, His tag  pdf  pdf  pdf


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GA4-M5247-100ug
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GA4-M5247-1mg (100ug x 10)
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Synonym

LGALS4,L36LBP,Gal-4,Antigen NY-CO-27,Lactose-binding lectin 4

Source

Mouse Galectin-4, His tag (GA4-M5247) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ile 326 (Accession # Q8K419-1).

Predicted N-terminus: His

Molecular Characterization

Poly-his
Galectin-4(Met 1 - Ile 326)Q8K419-1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 38.6 kDa. The protein migrates as 44 kDa and 45-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Mouse Galectin-4, His tag (Cat. No. GA4-M5247) SDS-PAGE gel

Mouse Galectin-4, His tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

Background

Galectin-4 (Gal-4), also known as L36LBP (L-36 lactose-binding protein). The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-4 expression is concentrated within microvilli in the gastrointestinal epithelium, where it can interact with CD3 and bind activated T cells in the lamina propria during intestinal inflammation. LGALS4 is an S-type lectin that is strongly underexpressed in colorectal cancer.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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