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Biotinylated Human EGFR, His Tag & Avi Tag (Avitag™)  pdf  pdf  pdf


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EGR-H82E3-25ug
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EGR-H82E3-200ug
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Synonym

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

Source

Biotinylated Human EGFR, His Tag (EGR-H82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).

Predicted N-terminus: Leu 25

Molecular Characterization

EGF R(Leu 25 - Ser 645)P00533-1
Poly-his
Avi

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 72.3 kDa. The protein migrates as 100-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 6 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Human EGFR, His Tag (Cat. No. EGR-H82E3) SDS-PAGE gel

Biotinylated Human EGFR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

Bioactivity

Biotinylated Human EGFR, His Tag (Cat. No. EGR-H82E3) ELISA bioactivity

Immobilized Anti-EGF R, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His Tag, Avi Tag (Cat. No. EGR-H82E3) with a linear range of 2-6 ng/mL (QC tested).

Request for protocol

Biotinylated Human EGFR, His Tag (Cat. No. EGR-H82E3) ELISA bioactivity

Immobilized Human EGF, Tag Free (Cat. No. EGF-H4114) at 10 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His Tag, Avi Tag (Cat. No. EGR-H82E3) with a linear range of 0.156-5 μg/mL (Routinely tested).

Request for protocol

 

Background

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


Limited Use License

The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

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