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Cynomolgus / Rhesus macaque CD47 Protein, His Tag  pdf  pdf  pdf


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Synonym

CD47,MER6,IAP,OA3

Source

Cynomolgus / Rhesus macaque CD47, His Tag (CD7-C52H1) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Glu 141 (Accession # NP_001253446.1).

Predicted N-terminus: Gln 19

Molecular Characterization

CD47(Gln 19 - Glu 141)NP_001253446.1
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 15.8 kDa. The protein migrates as 30-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Cynomolgus / Rhesus macaque CD47, His Tag (Cat. No. CD7-C52H1) SDS-PAGE gel

Cynomolgus / Rhesus macaque CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Bioactivity

Cynomolgus / Rhesus macaque CD47, His Tag (Cat. No. CD7-C52H1) ELISA bioactivity

Immobilized Human SIRP alpha, Fc Tag (Cat. No. SIA-H5251) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque CD47, His Tag (Cat. No. CD7-C52H1) with a linear range of 4-63 ng/mL (QC tested).

Request for protocol

Cynomolgus / Rhesus macaque CD47, His Tag (Cat. No. CD7-C52H1) ELISA bioactivity

Immobilized Anti-CD47, Human IgG4 at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque CD47, His Tag (Cat. No. CD7-C52H1) with a linear range of 0.2-5 ng/mL (Routinely tested).

Request for protocol

 

Background

Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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