Browse Products By Molecule:

This web search service is supported by Google Inc.

Products --> Recombinant Proteins

Human Integrin alpha E beta 7 (ITGAE&ITGB7) Heterodimer Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
IT7-H52W7-50ug
    $350.00
In-stock
Place Purchase Orders


Synonym

Integrin alpha E beta 7,ITGAE&ITGB7,HML-1,CD103

Source

Human ITGAE & ITGB7 Heterodimer Protein (IT7-H52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Ser 1124 (ITGAE) & Glu 20 - His 723 (ITGB7) (Accession # P38570-1 (ITGAE) & P26010-1 (ITGB7)).

Predicted N-terminus: Phe 19 (ITGAE) & Glu 20 (ITGB7)

Molecular Characterization

ITGAE(Phe 19 - Ser 1124 )P38570-1
Acidic Tail
His
ITGB7(Glu 20 - His 723 )P26010-1
Basic Tail

Human ITGAE & ITGB7 Heterodimer Protein, produced by co-expression of ITGAE and ITGB7, has a calculated MW of 128.6 kDa (ITGAE) and 81.8 kDa (ITGB7). Subunit ITGAE is fused with a polyhistidine tag at the C-terminus and subunit ITGB7 contains no tag. The predicted N-terminus is Phe 19 (ITGAE) & Glu 20 (ITGB7). The reducing (R) protein migrates as 135-150 kDa (ITGAE) and 105-115 kDa (ITGB7) respectively due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human ITGAE & ITGB7 Heterodimer Protein (Cat. No. IT7-H52W7) SDS-PAGE gel

Human ITGAE & ITGB7 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity

Human ITGAE & ITGB7 Heterodimer Protein (Cat. No. IT7-H52W7) ELISA bioactivity

Immobilized Human ITGAE&ITGB7, His Tag (Cat. No. IT7-H52W7) at 5 μg/mL (100 μL/well) can bind Human E-Cadherin, Fc Tag (Cat. No. ECD-H5250) with a linear range of 0.08-2.5 μg/mL (QC tested).

Request for protocol

 

Background

Integrin alpha E beta 7 consist of two major subunits: Integrin alpha E (ITGAE) also known as CD103 (cluster of differentiation 103) and Integrin beta-7 is an integrin protein that in humans is encoded by the ITGB7 gene. Integrin alpha E beta 7 (CD103) is expressed mainly by cells of the T lymphocyte lineage within mucosal tissues. This is a strikingly narrow pattern of expression compared with that of other integrins. Lymphocytes expressing alpha E beta 7 are abundant in the gut and comprise a major part of the total T cell complement of the body. The effectiveness of lung transplantation is marred by the relatively high incidence of rejection. The lung normally contains a large population of lymphocytes in contact with the airway epithelium, a proportion of which expresses the mucosal integrin, alpha(E)(CD103)beta(7). This integrin is not a homing receptor, but is thought to retain lymphocytes at the epithelial surface.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Questions & Comments