Browse Products By Molecule:

This web search service is supported by Google Inc.

Products --> Enzymes

Human PLAU / uPA Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
PLU-H5229-50ug
    $310.00
PLU-H5229-1mg (250ug x 4)
    $1800.00
In-stock
Place Purchase Orders


Synonym

Urokinase,PLAU,ATF,UPA,URK,u-PA,BDPLT5,QPD

Source

Human PLAU, His Tag (PLU-H5229) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Leu 431 (Accession # NP_002649.1).

Predicted N-terminus: Ser21, Ile179 & Lys156 

Molecular Characterization

PLAU(Ser 21 - Leu 431)NP_002649.1
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 45 kDa. The protein migrates as four bands corresponding to the short α chain, long α chain, β chain and unprocessed full-length chain with the molecular mass of 20 kDa, 23kDa, 32 kDa and 50 kDa respectively under reducing (R) condition (SDS-PAGE) due to glycosylation and cleavage.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Sodium Acetate, 100 mM NaCl, pH5.0. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human PLAU, His Tag (Cat. No. PLU-H5229) SDS-PAGE gel

Human PLAU, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

Background

Urokinase - type plasminogen activator is also known as PLAU and UPA, a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys – Pro – Gly - Arg560 - Val561 – Val – Gly – Gly – Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction).

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


Related Products