Human PLAU, His Tag (PLU-H5229) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Leu 431 (Accession # NP_002649.1).
Predicted N-terminus: Ser21, Ile179 & Lys156
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 45 kDa. The protein migrates as four bands corresponding to the short α chain, long α chain, β chain and unprocessed full-length chain with the molecular mass of 20 kDa, 23kDa, 32 kDa and 50 kDa respectively under reducing (R) condition (SDS-PAGE) due to glycosylation and cleavage.
Less than 1.0 EU per μg by the LAL method.
>92% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM NaAc, 100 mM NaCl, pH5.0. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.