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Human B7-H2 / ICOSLG Protein  pdf  pdf  pdf


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B72-H5221-200ug
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B72-H5221-1mg (500ug x 2)
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Synonym

ICOSLG,B7-H2,B7H2,B7RP-1,B7RP1,CD275,GL50,ICOS-L,ICOSL,LICOS,ICOS ligand

Source

Human B7-H2 / ICOSLG Protein, Asp 19 - Ser 258 (Accession # NP_056074) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

B7-H2 (Asp 19-Ser 258)NP_056074

polyhistidine

Human B7-H2, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 27.5 kDa. The predicted N-terminus is Asp 19. The reducing (R) protein migrates as 35-60 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE

Human B7-H2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Immobilized Human B7-H2, His Tag (Cat# B72-H5221) at 1 μg/mL (100 µl/well),can bind Human ICOS, Fc Tag (Cat# ICS-H5250) with a linear of 0.07-2.3 ng/mL.

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Background

ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.

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References