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Human Bcl-x / BCL2L1 Protein  pdf  pdf  pdf


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BC1-H5120-100ug
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Synonym

BCL2L1,Bcl-XL,Bcl2-L-1,BCL-XL/S,BCL2L,BCLX,BCLXL,BCLXS,Bcl-X,bcl-xS

Source

Human Bcl-x, His Tag (BC1-H5120) is expressed from E.coli cells. It contains AA Met 1 - Arg 212 (Accession # AAH19307).

Predicted N-terminus: Met 1

Molecular Characterization

Bcl-x(Met 1 - Arg 212)AAH19307
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 24.6 kDa. The protein migrates as 26 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 20mM HEPES, 50mM KCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Bcl-x, His Tag (Catalog # BC1-H5120) SDS-PAGE gel

Human Bcl-x, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

Background

Bcl-2-like protein 1 (BCL2L1), is also known as Apoptosis regulator Bcl-X (Bcl-XL). BCL2L1 / Bcl-XL is a homodimer protein and belongs to the Bcl-2 family. BCL2L1 is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. BCL2L1 is a potent inhibitor of cell death. BCL2L1 Inhibits activation of caspases. BCL2L1 appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. BCL2L1 / Bcl-XL also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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