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Human Semaphorin 4A / SEMA4A Protein  pdf  pdf  pdf


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Synonym

SEMA4A,SEMAB,SEMB,Semaphorin-4A,Semaphorin-B

Source

Human Semaphorin 4A / SEMA4A Protein (Human Semaphorin 4A, His Tag) Gly 33 - His 683 (Accession # AAH20974) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Human Semaphorin 4A, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 72.7 kDa. The predicted N-terminus is Gly 33. The reducing (R) protein migrates as 70-80 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE


Human Semaphorin 4A / SEMA4A Protein
Human Semaphorin 4A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
 
 

Citations

Background

Semaphorin-4A (SEMA4A) is also known as Semaphorin-B (SEMAB), which belongs to the semaphorin family. SEMA4A contains one Ig-like C2-type (immunoglobulin-like) domain, one PSI domain and one sema domain. SEMA4A is cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. SEMA4A plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. SEMA4A also promotes phosphorylation of TIMD2 and inhibits angiogenesis. SEMA4A promotes axon growth cone collapse and inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

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References