Browse Products By Molecule:

This web search service is supported by Google Inc.

Products --> Other Recombinant Proteins

Human Semaphorin 4A / SEMA4A Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
SEA-H5224-100ug
    $260.00
SEA-H5224-1mg (500ug x 2)
    $1600.00
In-stock
Place Purchase Orders


Synonym

SEMA4A,SEMAB,SEMB,Semaphorin-4A,Semaphorin-B

Source

Human Semaphorin 4A, His Tag (SEA-H5224) is expressed from human 293 cells (HEK293). It contains AA Gly 33 - His 683 (Accession # AAH20974).

Predicted N-terminus: Gly 33

Molecular Characterization

SEMA4A(Gly 33 - His 683)AAH20974
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 72.7 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Semaphorin 4A, His Tag (Cat. No. SEA-H5224) SDS-PAGE gel

Human Semaphorin 4A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Citations

Background

Semaphorin-4A (SEMA4A) is also known as Semaphorin-B (SEMAB), which belongs to the semaphorin family. SEMA4A contains one Ig-like C2-type (immunoglobulin-like) domain, one PSI domain and one sema domain. SEMA4A is cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. SEMA4A plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. SEMA4A also promotes phosphorylation of TIMD2 and inhibits angiogenesis. SEMA4A promotes axon growth cone collapse and inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.