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Human ENPP-2 / Autotaxin Protein  pdf  pdf  pdf


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EN2-H5241-50ug
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EN2-H5241-250ug
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Synonym

ENPP2, E-NPP 2, Autotaxin, LysoPLD, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA

Source

Human ENPP-2, His Tag (EN2-H5241) is expressed from human 293 cells (HEK293). It contains AA Asp 49 - Ile 863 (Accession # NP_001035181).

Predicted N-terminus: His

Molecular Characterization

Poly-his
ENPP-2(Asp 49 - Ile 863)NP_001035181

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 94.5 kDa. The protein migrates as 120-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human ENPP-2, His Tag (Cat. No. EN2-H5241) SDS-PAGE gel

Human ENPP-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

Background

Autotaxin also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (E-NPP 2 or ENPP2), Extracellular lysophospholipase D (LysoPLD), Autotaxin (ATX or ATX-X), which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP2 contains two SMB (somatomedin-B) domains. ENPP2 hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. ENPP2 can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. ENPP2 stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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