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Your Position: Home > Protein > Spike protein > SPN-S52H6

SARS S protein (R667A, K968P, V969P), His Tag (MALS verified)

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  • Synonym
    Spike,S protein,Spike glycoprotein,S glycoprotein
  • Source
    SARS S protein (R667A, K968P, V969P), His Tag (SPN-S52H6) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P)). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.
    Predicted N-terminus: Ser 14
  • Molecular Characterization
    Spike protein Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike protein SDS-PAGE

SARS S protein (R667A, K968P, V969P), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Spike protein MALS images

The purity of SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) is more than 90% and the molecular weight of this protein is around 508-562 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Spike protein ELISA

Immobilized SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-6 ng/mL (QC tested).

 Spike protein ELISA

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) with a linear range of 2-20 ng/mL (Routinely tested).

 Spike protein ELISA

Immobilized Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) with a linear range of 5-78 ng/mL (Routinely tested).

 Spike protein ELISA

Immobilized SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (Routinely tested).

  • Background
    The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates
  • Purchaser Notification
    Important Licensing Information: This product may be covered by Limited Use Label License(s). By use of this product, you accept the terms and conditions of all applicable Limited Use Label License(s).
  • Limited Use Label License No. 17: Prefusion Coronavirus Spike Proteins
    This product and its use is the subject of one or more U.S. Provisional Patent Application Nos. 62/412,703; 62/972,886; U.S. Patent Application No. 16/344,774 and corresponding patents and applications in other countries. This product is sold under licenses from The National Institute of Allergy & Infectious Diseases/National Institutes of Health. Rights to use this product are limited to research only (including Non-clinical Diagnostic Kits), and expressly exclude the right to use in human therapeutics, vaccines and clinical diagnostics and any use involving the administration of this product to humans.

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