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Your Position: Home > Protein > FOLR3 > FO3-P52H3

Papio anubis FOLR3 (A104T) Protein, His Tag (MALS verified)

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  • Synonym
    folate receptor 3 (gamma),Folate receptor 3,folate receptor gamma,FOLR3,FR-G,FR-gamma,gamma-Hfr,
  • Source
    Papio anubis FOLR3 (A104T) Protein, His Tag(FO3-P52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - Ser 245 (Accession # XP_003910408.1 (A104T)).
    Predicted N-terminus: Ala 22
  • Molecular Characterization
    FOLR3 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.5 kDa. The protein migrates as 33-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100mM Glycine, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FOLR3 SDS-PAGE

Papio anubis FOLR3 (A104T) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
FOLR3 MALS images

The purity of Papio anubis FOLR3 (A104T) Protein, His Tag (Cat. No. FO3-P52H3) is more than 95% and the molecular weight of this protein is around 35-50 kDa verified by SEC-MALS.

Bioactivity-ELISA
 FOLR3 ELISA

Immobilized Folic acid -BSA at 5 μg/mL (100 μL/well) can bind Papio anubis FOLR3 (A104T) Protein, His Tag (Cat. No. FO3-P52H3) with a linear range of 2-20 ng/mL (QC tested).

  • Background
    FOLR3, also know as folate receptor gamma, is a member of FRs. Folic acid and its reduced derivatives are transported via two widely expressed transporters, the reduced folate carrier (RFC) and the proton-coupled folate transporter (PCFT), and via a family of glycosyl-phosphatidylinositol (GPI)-anchored receptors with limited expression profiles known as folate receptors (FRs).Folate receptors (FR) are high affinity receptors that transport folate via endocytosis. Human FR exists in three isoforms, alpha and beta equipped with GPI anchors, and gamma not possessing a GPI anchor.
  • Clinical and Translational Updates

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