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Your Position: Home > Protein > HLA-G > HLG-H51H3

Human HLA-G protein, His Tag

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  • Synonym
    HLA-G, HLA class I histocompatibility antigen, alpha chain G, HLAG, HLA-6.0
  • Source
    Human HLA-G protein, His Tag(HLG-H51H3) is expressed from E. coli cells. It contains AA Gly 25 - Ala 206 (Accession # P17693-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    HLA-G Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 23 kDa. The protein migrates as 23-26 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, 0.5 M NaCl, pH 7.3 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-G SDS-PAGE

Human HLA-G protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 HLA-G ELISA

Immobilized Human HLA-G protein, His Tag (Cat. No. HLG-H51H3) at 5 μg/mL (100 μL/well) can bind Human KIR2DL4, Fc Tag (Cat. No. KI4-H5255) with a linear range of 1.6-50 ng/mL (QC tested).

 HLA-G ELISA

Immobilized Human HLA-G protein, His Tag (Cat. No. HLG-H51H3) at 5 μg/mL (100 μL/well) can bind Human LILRB2, Fc Tag (Cat. No. LI2-H5253) with a linear range of 0.3-2 ng/mL (Routinely tested).

  • Background
    Human leukocyte antigen-G (HLA-G) is a group of closely linked genes located on the short arm of human chromosome 6. Hla-g belongs to a non-classical major histocompatibility complex. MHC class I molecules are selectively highly expressed in extravvillous trophoblast cells invading the uterine decidual membrane.The gene structure of HLA-G is similar to that of HLA-A,HLA-B and HLA-C, but the termination code appears in advance so that the intracellular segment of protein product encoded by HLA-G is only 6 amino acids, which is significantly shorter than the 30 amino acids of classical HLA classI antigen. The Human HLA-G & B2M & RIIPRHLQL Complex Protein is a complex of HLA-G of the MHC Class I, B2M and RMFPNAPYL peptide.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $350.00

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