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Your Position: Home > ActiveMax® Cytokines > Human VEGF165 Protein,HEK293 expressed,ActiveMax

ActiveMax® Recombinant Human VEGF165 (HPLC-verified)

  • Synonym
    RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
  • Source
    ActiveMax® Human VEGF165 (VE5-H4210) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # NP_001165097).
    Predicted N-terminus: Ala 27
  • Molecular Characterization
    VEGF165(Ala 27 - Arg 191) NP_001165097

    This protein carries no "tag".

    The protein has a calculated MW of 19 kDa (monomer). As a result of glycosylation, the protein migrates as 24 kDa (monomer) under reducing (R) condition, and 43-50 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >98% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
ActiveMax® Human VEGF165 (Cat. No. VE5-H4210) SDS-PAGE gel

ActiveMax® Human VEGF165 on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

SEC-HPLC
ActiveMax® Human VEGF165 (Cat. No. ) HPLC images

A SEC-HPLC analysis showing 95% of ActiveMax® Human VEGF165 (VE5-H4210) present as active homodimers.

Bioactivity
ActiveMax® Human VEGF165ActiveMax® Human VEGF165 (Cat. No. VE5-H4210) ELISA bioactivity

Immobilized Human VEGF165, Tag Free (Cat. No. VE5-H4210) at 0.01 μg/mL (100 μL/well) can bind Human VEGF R1, His Tag (Cat. No. VE1-H5220) with a linear range of 4-31 ng/mL (QC tested).

ActiveMax® Human VEGF165ActiveMax® Human VEGF165 (Cat. No. VE5-H4210) ELISA bioactivity

Immobilized Human VEGF165, Tag Free (Cat. No. VE5-H4210) at 0.01 μg/mL (100 μL/well) can bind VEGFR1/R2-Fc with a linear range of 0.4-6 ng/mL (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Immobilized VEGF165 (Cat.No. VE5-H4210) on CM5 Chip, can bind Avastin with an affinity constant of 0.641 nM as determined in SPR assay (Biacore T200) ( Routinely tested).

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Price(USD) : $335.00

Inventory : In stock

Price(USD) : $3500.00

Inventory : In stock

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