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Your Position: Home > All Other Proteins > CD4 > Human CD4 /LEU3 Protein

Human CD4 Protein

  • Synonym
    CD4,CD4mut,LEU3
  • Source
    Human CD4, His Tag (LE3-H5228) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Trp 390 (Accession # AAH25782).
    Predicted N-terminus: Lys 26
  • Molecular Characterization
    CD4(Lys 26 - Trp 390) AAH25782

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 41.5 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD4, His Tag (Cat. No. LE3-H5228) SDS-PAGE gel

Human CD4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity
Human CD4, His TagHuman CD4, His Tag (Cat. No. LE3-H5228) ELISA bioactivity

Immobilized Human CD4, His Tag (Cat. No. LE3-H5228) at 5 μg/mL (100 μL/well) can bind Biotinylated Human GP120 (HIV) (Cat. No. GP5-V182E9) with a linear range of 0.039-0.625 μg/mL (QC tested).

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