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Your Position: Home > Immune Checkpoint Proteins > PD-1 > Mouse PD1 /PDCD1 Protein, With C-Fc Tag

Mouse PD-1 / PDCD1 Protein, Fc Tag

  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    Mouse PD-1, Fc Tag (PD1-M5259) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_032824).
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    Online(Leu 25 - Gln 167) NP_032824

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 43.2 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) SDS-PAGE gel

Mouse PD-1, Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Mouse PD-1, Fc TagMouse PD-1, Fc Tag (Cat. No. PD1-M5259) ELISA bioactivity

Immobilized Mouse PD-L1, His Tag (Cat. No. PD1-M5220) at 10 μg/mL (100 μl/well) can bind Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) with a linear range of 0.16-5 μg/mL (QC tested).

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