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Your Position: Home > All Other Proteins > IL-2 R alpha > Human IL2RA /CD25 Protein

Human IL-2 R alpha / CD25 Protein

  • Synonym
    IL2RA,CD25,p55,IL2-RA,IL-2-RA
  • Source
    Human IL-2 R alpha, His Tag (ILA-H52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408).
    Predicted N-terminus: Glu 22
  • Molecular Characterization
    IL-2 R alpha(Glu 22 - Cys 213) NP_000408

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 23.6 kDa. The protein migrates as 34-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) SDS-PAGE gel

Human IL-2 R alpha, His Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human IL-2 R alpha, His TagHuman IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) ELISA bioactivity

Immobilized Human IL-2, Tag Free (Cat. No. IL2-H4216) at 5μg/mL (100 μL/well) can bind Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) with a linear range of 0.01-1.11 μg/mL (QC tested).

  • Background
    Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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