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Your Position: Home > MABSol® Biotin Labeled Proteins > Biotinylated Human FcRN & B2M Heterodimer Protein,Avi Tag

Biotinylated Human FcRn/FCGRT & B2M, Avi Tag (Avitag™)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    MABSol® Biotinylated Human FcRn / FCGRT & B2M Heterodimer Protein (FCM-H82W4) is expressed from human HEK293 cells. It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # NP_001129491.1 (FCGRT) & AAH32589 (B2M)).
    Predicted N-terminus: Ala 24(FCGRT) & Ile 21(B2M)
  • Molecular Characterization
    FcRn(Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) NP_001129491.1 (FCGRT) & AAH32589 (B2M)

    The subunit FCGRT carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The subunit Beta-2 microglobulin (B2M) carries a Strep II tag at the C-terminus. The protein has a calculated MW of 33 kDa (FCGRT), 13.1 kDa (B2M) . The protein migrates as 36 kDa (FCGRT), 14 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human FcRn / FCGRT & B2M (Cat. No. FCM-H82W4) SDS-PAGE gel

Biotinylated Human FcRn / FCGRT & B2M on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Biotinylated Human FcRn / FCGRT & B2MBiotinylated Human FcRn / FCGRT & B2M (Cat. No. FCM-H82W4) ELISA bioactivity

Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Human FcRn / FCGRT & B2M (Cat. No. FCM-H82W4 ) with a linear range of 0.02-1.25 μg/mL (QC tested).

Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Human FcRn / FCGRT & B2M, Avi Tag (Avitag™) (Cat.# FCM-H82W4) coupled to SA coated sensor chip can bind trastuzumab with an affinity constant of 2.2-2.6 μM as determined in a SPR assay (Biacore 2000). The data is generally provided by Biaffin GmbH &Co KG, Germany (Routinely tested).

  • Background
    FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Inventory : In stock

Price(USD) : $1380.00

Inventory : In stock

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