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Your Position: Home > Immune Checkpoint Proteins > PD-1 > PD1-R5253

Rat PD-1 / PDCD1 Protein, Fc Tag

  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    Rat PD-1, Fc Tag (PD1-R5253) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_001100397.1).
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    Online(Leu 25 - Gln 167) NP_001100397.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 42.9 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rat PD-1, Fc Tag (Cat. No. PD1-R5253) SDS-PAGE gel

Rat PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Rat PD-1, Fc TagRat PD-1, Fc Tag (Cat. No. PD1-R5253) ELISA bioactivity

Immobilized Mouse PD-L2, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. PD2-M52A5) at 2 μg/mL (100 μL/well) can bind Rat PD-1, Fc Tag (Cat. No. PD1-R5253) with a linear range of 2-20 ng/mL (QC tested).

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