Save Product
Product Details
Synonyms
CAIX, CA9, CA-IX, G250, MN, P54, 58N, pMW1
Source
Human Carbonic Anhydrase IX (138-414), His Tag (CA9-H5220) is expressed from human 293 cells (HEK293). It contains AA Gln 138 - Asp 414 (Accession # NP_001207.2).
Predicted N-terminus: Gln 138
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 30.9 kDa. The protein migrates as 34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Human Carbonic Anhydrase IX (138-414), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human Carbonic Anhydrase IX (138-414), His Tag (Cat. No. CA9-H5220) is more than 95% and the molecular weight of this protein is around 60-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Carbonic Anhydrase IX (138-414), His Tag (Cat. No. CA9-H5220) at 1 μg/mL (100 μL/well) can bind Anti-Carbonic Anhydrase IX Antibody, Human IgG1 (Cat. No. AM213) with a linear range of 0.3-1.2 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-Carbonic Anhydrase IX Antibody captured on Protein A Chip can bind Human Carbonic Anhydrase IX (138-414), His Tag (Cat. No. CA9-H5220) with an affinity constant of 1.29 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.
Recent Advances
Drug Development Progress- English Name：
Carbonic anhydrase IX
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
16 Details
- Highest Development Stage：
Phase 3 Clinical
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
