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Your Position: Home > All Other Proteins > Integrin alpha V beta 1 > Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein

Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein

  • Synonym
    Integrin alpha V beta 1,ITGAV&ITGB1
  • Source
    Human ITGAV & ITGB1 Heterodimer Protein (IT1-H52E1) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1) (Accession # NP_002201 (ITGAV)& P05556 (ITGB1)).
    Predicted N-terminus: Phe 31 (ITGAV) & Gln 21 (ITGB1)
  • Molecular Characterization
    Online(Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1)) NP_002201 (ITGAV)& P05556 (ITGB1)

    Human ITGAV & ITGB1 Heterodimer Protein, produced by co-expression of ITGAV and ITGB1, has a calculated MW of 113 kDa (ITGAV) and 83.7 kDa (ITGB1). Subunit ITGAV is  fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Gln 21 (ITGB1). The reducing (R) protein migrates as 145 kDa (ITGAV) and 116 kDa (ITGB1) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,2 mM CaCl2,pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human ITGAV & ITGB1 Heterodimer Protein (Cat. No. IT1-H52E1) SDS-PAGE gel

Human ITGAV & ITGB1 Heterodimer Protein on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human ITGAV & ITGB1 Heterodimer ProteinHuman ITGAV & ITGB1 Heterodimer Protein (Cat. No. IT1-H52E1) ELISA bioactivity

Immobilized Fibronectin at 10 μg/mL (100 μL/well) can bind Human ITGAV & ITGB1 Heterodimer Protein (Cat. No. IT1-H52E1) with a linear range of 0.156-1.25 μg/mL (QC tested).

  • Background
    Integrin alpha-5/beta-1 is a receptor for ibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-V/beta-1 is also a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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