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Your Position: Home > All Other Proteins > Integrin alpha V beta 1 > Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein

Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein

  • Synonym
    Integrin alpha V beta 1,ITGAV&ITGB1
  • Source
    Human ITGAV & ITGB1 Heterodimer Protein (IT1-H52E1) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1) (Accession # NP_002201 (ITGAV)& P05556 (ITGB1)).
    Predicted N-terminus: Phe 31 (ITGAV) & Gln 21 (ITGB1)
  • Molecular Characterization
    ITGAV&ITGB1(Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1)) NP_002201 (ITGAV)& P05556 (ITGB1)

    Human ITGAV & ITGB1 Heterodimer Protein, produced by co-expression of ITGAV and ITGB1, has a calculated MW of 113 kDa (ITGAV) and 83.7 kDa (ITGB1). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Gln 21 (ITGB1). The reducing (R) protein migrates as 145 kDa (ITGAV) and 116 kDa (ITGB1) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGAV & ITGB1 Heterodimer Protein (Cat. No. IT1-H52E1) SDS-PAGE gel

Human ITGAV & ITGB1 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Integrin alpha-5/beta-1 is a receptor for ibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-V/beta-1 is also a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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