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Your Position: Home > Receptor Tyrosine Kinases > M-CSF R > CSR-C5252

Cynomolgus M-CSF R / CSF1R / CD115 Protein, Fc Tag

  • Synonym
    CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR
  • Source
    Cynomolgus M-CSF R, Fc Tag (CSR-C5252) is expressed from human 293 cells (HEK293). It contains AA Ile 99 - Pro 596 (Accession # G7P8P1).
    Predicted N-terminus: Ile 99
  • Molecular Characterization
    Online(Ile 99 - Pro 596) G7P8P1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 81.9 kDa. As a result of glycosylation, the protein migrates as 110-125 kDa under reducing (R) condition, and 200- 240 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus M-CSF R, Fc Tag (Cat. No. CSR-C5252) SDS-PAGE gel

Cynomolgus M-CSF R, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Cynomolgus M-CSF R, Fc TagCynomolgus M-CSF R, Fc Tag (Cat. No. CSR-C5252) ELISA bioactivity

Immobilized Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) at 2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Cynomolgus M-CSF R, Fc Tag (Cat. No. CSR-C5252) with a linear range of 1-39 ng/mL (QC tested).

Cynomolgus M-CSF R, Fc TagCynomolgus M-CSF R, Fc Tag (Cat. No. CSR-C5252) ELISA bioactivity

Immobilized Human M-CSF, His Tag (Cat. No. MCF-H5247) at 5 μg/mL (100 μL/well) can bind Cynomolgus M-CSF R, Fc Tag (Cat. No. CSR-C5252) with a linear range of 1-39 ng/mL (Routinely tested).

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