>Recombinant Human Activin A / INHBA Protein,Tag Free, low endotoxin
This protein carries no "tag".
The protein has a calculated MW of 13.0 kDa. the protein migrates as 14 kDa under reducing (R) condition, and 27 kDa under non-reducing (NR) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 0.056% TFA in 60% ACN. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
ActiveMax® Human Activin A,Tag Free, low endotoxin on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized ActiveMax® Human Activin A,Tag Free, low endotoxin (Cat. No. ACA-H421b) at 10μg/mL (100 μL/well) can bind Human ACVR1B, Fc Tag (Cat. No. ACB-H5251) with a linear range of 0.3-2.5 μg/mL (QC tested).
The bio-activity of ActiveMax® Human Activin A, Tag Free, low endotoxin (Cat. No. ACA-H421b) was determined by dose-dependent inhibition of the proliferation of MPC-11 cells. The EC50 for this effect is typically ≤ 9.5ng/ml (Routinely tested).
Authors: Basu M, et al.
Journal: Mol Cancer 2015
Application: Cell Culture
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