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Your Position: Home > Immune Checkpoint Proteins > CD30 > CD0-H82E6

Biotinylated Human CD30 / TNFRSF8 Protein, Avitag™,His Tag

  • Synonym
    TNFRSF8,CD30,D1S166E,Ki-1
  • Source
    Biotinylated Human CD30, Avitag,His Tag (CD0-H82E6) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2).
    Predicted N-terminus: Phe 19
  • Molecular Characterization
    Online(Phe 19 - Lys 379) NP_001234.2

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 41.1 kDa. The protein migrates as 60-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD30, Avitag,His Tag (Cat. No. CD0-H82E6) SDS-PAGE gel

Biotinylated Human CD30, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human CD30, Avitag,His TagBiotinylated Human CD30, Avitag,His Tag (Cat. No. CD0-H82E6) ELISA bioactivity

Immobilized Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD30, Avitag,His Tag (Cat. No. CD0-H82E6) with a linear range of 10-78 ng/mL (QC tested).

Biotinylated Human CD30, Avitag,His TagBiotinylated Human CD30, Avitag,His Tag (Cat. No. CD0-H82E6) ELISA bioactivity

Immobilized Anti-CD30 MAb, Human IgG1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD30, Avitag,His Tag (Cat. No. CD0-H82E6) with a linear range of 0.4-6.5 ng/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human CD30, Avitag,His Tag (Cat. No. CD0-H82E6) on SA Biosensor, can bind Human CD30 Ligand, His Tag, low endotoxin (Cat. No. CDL-H524b) with an affinity constant of 132 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

Promotion & Exhibitions

  • PEGS Boston 2019
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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:17 Details
  • Latest Research Phase:Approved

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