This protein carries an Avi tag at the C-terminus.
The protein has a calculated MW of 28.4 kDa. The protein migrates as 32-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human IgG1 Fc, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Biotinylated Human IgG1 Fc, Avitag (Cat. No. IG1-H8213) with an affinity constant of 0.377 μM as determined in SPR assay (Biacore 8K) (Routinely tested).
Authors: Mukundan S, et al.
Journal: Am J Adv Drug Deliv 2018
Application: Flow Cytometry
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