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Your Position: Home > Receptor Tyrosine Kinases > EGF R > Biotinylated Human EGF R, His Tag, Avi Tag

Biotinylated Human EGFR, His Tag & Avi Tag (Avitag™)

  • Synonym
    EGFR,ERBB,ERBB1,HER1,PIG61,mENA
  • Source
    Biotinylated Human EGFR, His Tag (EGR-H82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    EGF R(Leu 25 - Ser 645) P00533-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 72.3 kDa. The protein migrates as 100-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 6 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human EGFR, His Tag (Cat. No. EGR-H82E3) SDS-PAGE gel

Biotinylated Human EGFR, His Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human EGFR, His TagBiotinylated Human EGFR, His Tag (Cat. No. EGR-H82E3) ELISA bioactivity

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His Tag, Avi Tag (Cat. No. EGR-H82E3) with a linear range of 2-6 ng/mL (QC tested).

Biotinylated Human EGFR, His TagBiotinylated Human EGFR, His Tag (Cat. No. EGR-H82E3) ELISA bioactivity

Immobilized Human EGF, Tag Free (Cat. No. EGF-H4114) at 10 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His Tag, Avi Tag (Cat. No. EGR-H82E3) with a linear range of 0.156-5 μg/mL (Routinely tested).

Evaluation of CAR expression
FACS Analysis of biotinylated EGFR binding cell surface anti-EGFR scFv
Evaluation of CAR expression

Cells were transfected with anti-EGFR scFv and cultured for 3 days. Three days post-transfection, 1e6 cells were stained for anti-EGFR scFv with Biotinylated Human EGFR, His Tag, Avi Tag (Cat. No. EGR-H82E3) followed by PE-conjugated streptavidin. Flow Cytometry assay shows that Biotinylated Human EGFR, His Tag, Avi Tag (Cat. No. EGR-H82E3) can bind to cells expressing anti-EGFR scFv. The concentration of EGFR used is 0.8 µg/mL. (Data are kindly provided by Guangzhou Bio-gene Technology Co. Ltd.)

  • Citations
  • Background
    The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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