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Your Position: Home > All Other Proteins > IL-7 R alpha > Human IL-7 R alpha / CD127 Protein, Mouse IgG2a Fc Tag

Human IL-7 R alpha / CD127 Protein, Mouse IgG2a Fc Tag

  • Synonym
    IL7Ra,CD127
  • Source
    Human IL-7 R alpha, Mouse IgG2a Fc tag (IL7-H5258) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Gly 236 (Accession # P16871-1).
    Predicted N-terminus: Glu 21
  • Molecular Characterization
    Online(Glu 21 - Gly 236) P16871-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 52.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human IL-7 R alpha, Mouse IgG2a Fc tag (Cat. No. IL7-H5258) SDS-PAGE gel

Human IL-7 R alpha, Mouse IgG2a Fc tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human IL-7 R alpha, Mouse IgG2a Fc tagHuman IL-7 R alpha, Mouse IgG2a Fc tag (Cat. No. IL7-H5258) ELISA bioactivity

Immobilized ActiveMax® Human IL-7, Tag Free (Cat. No. IL7-H4219) at 2 μg/mL (100 μL/well) can bind Human IL-7 R alpha, Mouse IgG2a Fc tag (Cat. No. IL7-H5258) with a linear range of 4-31 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Captured Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) on CM5 chip via anti-mouse antibodies surface can bind ActiveMax® Human IL-7, Tag Free (Cat. No. IL7-H4219) with an affinity constant of 13.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

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