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Your Position: Home > Fc Receptor Proteins > FcRn (FCGRT & B2M) > FCN-F82W3

Biotinylated Feline FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™ (SPR verified)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    Biotinylated Feline FCGRT&B2M Heterodimer Protein, His,Avitag (SPR verified) (FCN-F82W3) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Val 21 - Met 118 (B2M) (Accession # W5VRL5-1 (FCGRT) & Q5MGS7-1 (B2M)).
    Predicted N-terminus: Ala 24 (FCGRT) & Val 21 (B2M)
  • Molecular Characterization
    Online(Ala 24 - Ser 297 (FCGRT) & Val 21 - Met 118 (B2M)) W5VRL5-1 (FCGRT) & Q5MGS7-1 (B2M)

    Biotinylated Feline FCGRT&B2M Heterodimer Protein, His,Avitag (SPR verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.0 kDa (FCGRT) and 11.5 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag. The reducing (R) protein migrates as 36-40 kDa (FCGRT) and 18-20 kDa (B2M) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Feline FCGRT&B2M Heterodimer Protein, His,Avitag (SPR verified) (Cat. No. FCN-F82W3) SDS-PAGE gel

Biotinylated Feline FCGRT&B2M Heterodimer Protein, His,Avitag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Feline FCGRT&B2M Heterodimer Protein, His,Avitag (SPR verified) (Cat. No. FCN-F82W3) captured on Biotin CAP-Series S Sensor Chip can bind Herceptin® with an affinity constant of 1.19 nM as determined in a SPR assay (Biacore T200) (QC tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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