0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Recombinant Proteins > VEGF R2 > Mouse VEGF R2 /KDR Protein, mouse IgG2a Fc Tag, low endotoxin

Mouse VEGF R2 / KDR Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    KDR,CD309,FLK1,VEGFR,VEGFR2
  • Source
    Mouse VEGF R2, mouse IgG2a Fc Tag (VE2-M5258) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 762 (Accession # P35918-1).
    Predicted N-terminus: Ala 20
  • Molecular Characterization
    VEGF R2(Ala 20 - Glu 762) P35918-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 110.1 kDa. The protein migrates as 120-135 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Mouse VEGF R2, mouse IgG2a Fc Tag (Cat. No. VE2-M5258) SDS-PAGE gel

Mouse VEGF R2, mouse IgG2a Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Mouse VEGF R2, mouse IgG2a Fc TagMouse VEGF R2, mouse IgG2a Fc Tag (Cat. No. VE2-M5258) ELISA bioactivity

Immobilized Human VEGF165, Tag Free (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2, mouse IgG2a Fc Tag (Cat. No. VE2-M5258) with a linear range of 1-31 ng/mL (QC tested).

Mouse VEGF R2, mouse IgG2a Fc TagMouse VEGF R2, mouse IgG2a Fc Tag (Cat. No. VE2-M5258) ELISA bioactivity

Immobilized Mouse VEGF120, Tag Free (Cat. No. VE0-M4211) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2, mouse IgG2a Fc Tag (Cat. No. VE2-M5258) with a linear range of 1-31 ng/mL (Routinely tested).

Mouse VEGF R2, mouse IgG2a Fc TagMouse VEGF R2, mouse IgG2a Fc Tag (Cat. No. VE2-M5258) ELISA bioactivity

Immobilized Mouse VEGF164, Tag Free (Cat. No. VE4-M4216) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2, mouse IgG2a Fc Tag (Cat. No. VE2-M5258) with a linear range of 2-31 ng/mL (Routinely tested).

  • Background
    Kinase insert domain receptor (KDR) is also known as CD309, FLK1, VEGFR, VEGFR2, and  is one of the subtypes of VEGFR. VEGF receptors are receptors for vascular endothelial growth factor (VEGF). There are three main subtypes of VEGFR, numbered 1, 2 and 3. The VEGF receptors have an extracellular portion consisting of 7 immunoglobulin-like domains, a single transmembrane spanning region and an intracellular portion containing a split tyrosine-kinase domain. VEGF-A binds to VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). VEGFR-2 appears to mediate almost all of the known cellular responses to VEGF.The function of VEGFR-1 is less well defined, although it is thought to modulate VEGFR-2 signaling. Another function of VEGFR-1 may be to act as a dummy/decoy receptor, sequestering VEGF from VEGFR-2 binding (this appears to be particularly important during vasculogenesis in the embryo). In addition, VEGFR2 is able to interact with HIV-1 extracellular Tat protein upon VEGF activation, and seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $300.00

Price(USD) : Inquire

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop