>All Other Proteins
>Biotinylated Human Glypican 3 / GPC3 Protein, His,Avitag
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 64.5 kDa. The protein migrates as 42 kDa(caused by a furin cleavage site) and 70-250 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>85% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Biotinylated Human Glypican 3, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Glypican 3, His,Avitag (Cat. No. GP3-H82E5) with a linear range of 1-16 ng/mL (QC tested).
293 cells were transfected with anti-GPC3-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human Glypican 3, His,Avitag (Cat. No. GP3-H82E5, 10 μg/mL) and C. biotinylated protein control, followed by FITC-conjugated streptavidin. A. Non-transfected 293 cells and C. biotinylated protein control were used as negative control. RFP was used to evaluate CAR (anti-GPC3-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human Glypican 3, His,Avitag (Cat. No. GP3-H82E5).
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Price(USD) : $350.00
Price(USD) : $1380.00
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