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Your Position: Home > All Other Proteins > Glypican 3 > GP3-H82E5

Biotinylated Human Glypican 3 / GPC3 Protein, His,Avitag™

  • Synonym
    GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1
  • Source
    Biotinylated Human Glypican 3, His,Avitag (GP3-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).
    Predicted N-terminus: Gln 25 & Ser 359
  • Molecular Characterization
    Online(Gln 25 - His 559) P51654-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 26.4 kDa respectively. The protein migrates as 40 kDa and 70-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Glypican 3, His,Avitag (Cat. No. GP3-H82E5) SDS-PAGE gel

Biotinylated Human Glypican 3, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity-ELISA
Biotinylated Human Glypican 3, His,AvitagBiotinylated Human Glypican 3, His,Avitag (Cat. No. GP3-H82E5) ELISA bioactivity

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Glypican 3, His,Avitag (Cat. No. GP3-H82E5) with a linear range of 1-16 ng/mL (QC tested).

Biotinylated Human Glypican 3, His,AvitagBiotinylated Human Glypican 3, His,Avitag (Cat. No. GP3-H82E5) ELISA bioactivity

Immobilized Anti-Human GPC3 Mab at 2 μg/mL (100 μL/well) can bind Biotinylated Human Glypican 3, His,Avitag (Cat. No. GP3-H82E5) with a linear range of 0.4-6 ng/mL (Routinely tested).

Evaluation of CAR expression
FACS Analysis of Anti-GPC3 CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-GPC3-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human Glypican 3, His,Avitag (Cat. No. GP3-H82E5, 10 μg/mL) and C. biotinylated protein control, followed by FITC-conjugated streptavidin. A. Non-transfected 293 cells and C. biotinylated protein control were used as negative control. RFP was used to evaluate CAR (anti-GPC3-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human Glypican 3, His,Avitag (Cat. No. GP3-H82E5).

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