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Your Position: Home > Recombinant Proteins > NTB-A > Biotinylated Human NTB-A / SLAMF6 Protein, His Tag, Avi Tag

Biotinylated Human NTB-A / SLAMF6 Protein, His Tag, Avi Tag (Avitag™)

  • Synonym
    NTB-A,SLAMF6,Ly108,NK-T-B-antigen,CD352,KALI
  • Source
    Biotinylated Human NTB-A, His Tag, Avi Tag (NTA-H82E6) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Met 226 (Accession # Q96DU3-1).
    Predicted N-terminus: Gln 22
  • Molecular Characterization
    NTB-A(Gln 22 - Met 226) Q96DU3-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 26.8 kDa. The protein migrates as 35-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human NTB-A, His Tag, Avi Tag (Cat. No. NTA-H82E6) SDS-PAGE gel

Biotinylated Human NTB-A, His Tag, Avi Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Biotinylated Human NTB-A, His Tag, Avi TagBiotinylated Human NTB-A, His Tag, Avi Tag (Cat. No. NTA-H82E6) ELISA bioactivity

Immobilized Human NTB-A (Cat. No. NTA-H52Ha) at 10 μg/mL (100 μL/well) can bind Biotinylated Human NTB-A, His Tag, Avi Tag (Cat. No. NTA-H82E6) with a linear range of 0.156-5 μg/mL (QC tested).

  • Background
    The SLAM family of receptors is composed of six members named SLAM (CD150); 2B4 (CD244); Ly-9 (CD229); CD84; natural killer, T and B cell antigen (NTB-A; Ly108 in the mouse. Most, if not all,immune cell types express more than one SLAM family receptor. All six SLAM-related genes are located within a 400–500 kilobase (kb) genomic segment on chromosome 1 in humans and mice. This feature implies that the genes that encode the SLAM family were created by serial gene duplication.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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