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Your Position: Home > Receptor Tyrosine Kinases > Insulin R > INR-H52Ha

Human Insulin R / CD220 (28-944) Protein, His Tag (SPR verified)

  • Synonym
    INSR,Insulin receptor,IR,CD220
  • Source
    Human Insulin R (28-944), His Tag (SPR verified) (INR-H52Ha) is expressed from human 293 cells (HEK293). It contains AA His 28 - Lys 944 (Accession # P06213-2).
    Predicted N-terminus: His 28
  • Molecular Characterization
    Online(His 28 - Lys 944) P06213-2

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 106.5 kDa. The protein migrates as 115-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Insulin R (28-944), His Tag (SPR verified) (Cat. No. INR-H52Ha) SDS-PAGE gel

Human Insulin R (28-944), His Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human Insulin R (28-944), His Tag (SPR verified)Human Insulin R (28-944), His Tag (SPR verified) (Cat. No. INR-H52Ha) ELISA bioactivity

Immobilized Human Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) at 5 μg/mL (100 μL/well)can bind Human IGF-II, Fc Tag (Cat. No. IG2-H4260) with a linear range of 0.078-1.25 μg/mL (QC tested).

Human Insulin R (28-944), His Tag (SPR verified)Human Insulin R (28-944), His Tag (SPR verified) (Cat. No. INR-H52Ha) ELISA bioactivity

Immobilized Human Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) at 5 μg/mL (100 μL/well)can bind Insulin Biotin Conjugate with a linear range of 0.02-0.313 μg/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) captured on CM5 chip via anti-His antibody, can bind Human Insulin with an affinity constant of 249 nM as determined in a SPR assay (Biacore T200) (QC tested).

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Price(USD) : $300.00

Price(USD) : $1980.00

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  • Number of Drugs in Clinical Trials:105 Details
  • Latest Research Phase:Approved

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