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Your Position: Home > Immune Checkpoint Proteins > CD30 ligand > CDL-H524b

Human CD30 Ligand / TNFSF8 Protein, His Tag, low endotoxin

  • Synonym
    CD30-L,CD153,TNFSF8,CD30L,CD30LG,CD153 antigen,CD30 antigen ligand,CD30 Ligand
  • Source
    Human CD30 Ligand, His Tag, low endotoxin (CDL-H524b) is expressed from human 293 cells (HEK293). It contains AA Gln 63 - Asp 234 (Accession # P32971-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Gln 63 - Asp 234) P32971-1

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 21.5 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD30 Ligand, His Tag, low endotoxin (Cat. No. CDL-H524b) SDS-PAGE gel

Human CD30 Ligand, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human CD30 Ligand, His Tag, low endotoxinHuman CD30 Ligand, His Tag, low endotoxin (Cat. No. CDL-H524b) ELISA bioactivity

Immobilized Human CD30, Fc Tag (Cat. No. CD0-H5250) at 2 μg/mL (100 μL/well) can bind Human CD30 Ligand, His Tag, low endotoxin (Cat. No. CDL-H524b) with a linear range of 0.039-0.313 μg/mL (QC tested).

Human CD30 Ligand, His Tag, low endotoxinHuman CD30 Ligand, His Tag, low endotoxin (Cat. No. CDL-H524b) ELISA bioactivity

Immobilized Human CD30 Ligand, His Tag, low endotoxin (Cat. No. CDL-H524b) at 2 μg/mL (100 μL/well) can bind Human CD30, Llama IgG2b Fc Tag, low endotoxin (Cat. No. TN8-H5250) with a linear range of 0.6-10 ng/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human CD30, Avitag,His Tag (Cat. No. CD0-H82E6) on SA Biosensor, can bind Human CD30 Ligand, His Tag, low endotoxin (Cat. No. CDL-H524b) with an affinity constant of 132 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that Human CD30 Ligand, His Tag, low endotoxin (Cat. No. CDL-H524b) can bind to K562-CD30 cells surface CD30. The concentration of Human CD30 Ligand is 3 μg/mL (Routinely tested).

FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that the binding of Human CD30 Ligand, His Tag, low endotoxin (Cat. No. CDL-H524b) to K562-CD30 cells surface CD30 was inhibited by increasing concentration of neutralizing anti-CD30 antibody. The concentration of Human CD30 Ligand used is 3 μg/mL. The IC50 is 0.2401 μg/mL (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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