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Your Position: Home > Fc Receptor Proteins > Fc gamma RIV / CD16-2 > Biotinylated Mouse Fc gamma RIV / CD16-2 Protein, His,Avitag (BLI verified)

Biotinylated Mouse Fc gamma RIV / CD16-2 Protein, His,Avitag™ (BLI verified)

  • Synonym
    Fc gamma RIV,CD16-2,Fcgr4
  • Source
    Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (FC4-M82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Gln 203 (Accession # A0A0B4J1G0-1).
    Predicted N-terminus: Gly 21
  • Molecular Characterization
    Online(Gly 21 - Gln 203) A0A0B4J1G0-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 24.6 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) SDS-PAGE gel

Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified)Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) ELISA bioactivity

Immobilized FMC63 at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) with a linear range of 0.3-20 ng/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) on SA Biosensor, can bind Rituximab with an affinity constant of 0.394 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

  • Background
    FcgR4(Low affinity immunoglobulin gamma Fc region receptor IV) is also known as CD16-2, FcgammaRIV, receptor for the Fc region of immunoglobulin gamma . Also acts as a receptor for the Fc region of immunoglobulin epsilon . Binds with intermediate affinity to both IgG2a and IgG2b . Does not display binding to IgG1 or IgG3. Plays a role in promoting bone resorption by enhancing osteoclast differentiation following binding to IgG2a. Binds with low affinity to both the a and b allotypes of IgE. Has also been shown to bind to IgE allotype a only but not to allotype b. Binding to IgE promotes macrophage-mediated phagocytosis, antigen presentation to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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