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Your Position: Home > Cytokines > Complement Component C5a > C5A-H525a

Human Complement Component C5a Protein, Fc Tag

  • Synonym
    C5a,Complement Component 5a
  • Source
    Human C5a, Fc Tag (C5A-H525a) is expressed from human 293 cells (HEK293). It contains AA Leu 679 - Arg 751 (Accession # P01031-1).
    Predicted N-terminus: Leu 679
  • Molecular Characterization
    Online(Leu 679 - Arg 751) P01031-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 34.6 kDa. The protein migrates as 38-40 kDa and 41-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human C5a, Fc Tag (Cat. No. C5A-H525a) SDS-PAGE gel

Human C5a, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human C5a, Fc TagHuman C5a, Fc Tag (Cat. No. C5A-H525a) ELISA bioactivity

Immobilized Human C5a, Fc Tag (Cat. No. C5A-H525a) at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 2-16 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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