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Your Position: Home > All Other Proteins > Fc epsilon RI alpha > FCA-H5259

Human Fc epsilon RI alpha Protein, Fc Tag (BLI verified)

  • Synonym
    FCER1A,FCE1A,FcERI
  • Source
    Human Fc epsilon RI alpha, Fc Tag (BLI verified) (FCA-H5259) is expressed from human 293 cells (HEK293). It contains AA Val 26 - Leu 204 (Accession # P12319-1).
    Predicted N-terminus: Val 26
  • Molecular Characterization
    Online(Val 26 - Leu 204) P12319-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 47.3 kDa. The protein migrates as 55-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Fc epsilon RI alpha, Fc Tag (BLI verified) (Cat. No. FCA-H5259) SDS-PAGE gel

Human Fc epsilon RI alpha, Fc Tag (BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human Fc epsilon RI alpha, Fc Tag (BLI verified) (Cat. No. FCA-H5259) on AHC Biosensor, can bind Immunoglobulin E, Human Plasma with an affinity constant of 0.188 nM as determined in BLI assay (ForteBio Octet Red96e) (QC Tested).

  • Background
    High affinity immunoglobulin epsilon receptor subunit alpha (FCER1A) is also known as Fc-epsilon RI-alpha (FcERI), IgE Fc receptor subunit alpha, FCE1A. FCER1A contains two Ig-like (immunoglobulin-like) domains. FCER1A binds to the Fc region of immunoglobulins epsilon and is a high affinity receptor. FCER1A is responsible for initiating the allergic response, which binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. FCER1A plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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