|Plate Blocking:||2% BSA Blocking Buffer|
|Formulations||Clear, 96-well plates, coated with 100uL of Streptavidin and blocked with 200uL of 2% BSA Blocking Buffer|
|Type||Detection Plate, Immunoassay, ELISA|
The Streptavidin Coated Plates, Clear, 96-Well is pre-coated with Streptavidin tetramer protein and blocked with BSA, it is ready-to-use polystyrene plate, which can be used for binding biotinylated proteins and antibodies, or probes for ELISA and other target-specific assays.
Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. The Streptavidin Coated Plates we provide are easy to use and widely available for applications.
Example ELISA Procedure
Your experiment will include 5 simple steps:
a) Add your Biotinylated sample to the plate, incubation for 1 hour and wash the plate.
b) Add your sample to the plate, incubation for 1 hour and wash the plate.
c) Add a diluted Detection antibody to the plate and incubation.
d) Wash the plate and next add TMB or other colorimetric HRP substrate.
e) Stop the substrate reaction by add diluted acid, and Read OD at 450 nm, the OD Value reflects the amount of antibody bound.
This Streptavidin Coated Plate is intended for Immunoassay and ELISA.
Flexible experimental design because of the plate consist with detachable 8-well strips.
Save your experiment time by pre-coated and blocked.
Easy and gentle immobilization of biotin-containing conjugates
No denaturing of the protein component of a conjugate upon binding
Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene.
Upon receipt store plates at 4°C in unopened pouches.
Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.
Plates are shipped at ambient temperature.
Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Anti-FMC63 antibody with a linear range of 0.1-3 ng/mL (QC tested).
Immobilized Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Human PD-L1, mFc Tag with a linear range of 0.4-6.25 ng/mL (QC tested).
Immobilized Biotinylated Human CD20 Full Length, His,Avitag (HEK293) (Cat. No. CD0-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Rituximab with a linear range of 78-625 ng/mL (QC tested).
Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Ipilimumab with a linear range of 78-1250 ng/mL (QC tested).
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