>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-BM1) is more than 95% and the molecular weight of this protein is around 135-170 kDa verified by SEC-MALS.
Measured by its binding ability in a functional ELISA. Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-BM1) with a linear range of 1-39 ng/mL (QC tested).
2e5 of Anti-CD19 CAR-293 cells were stained with a series of concentrations of Biotinylated Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (QC tested).
5e5 of PBMCs were stained with a series of concentrations of Biotinylated Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (QC tested).
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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