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Your Position: Home > Viral Proteins > Spike protein > SPN-H82E3

Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His,Avitag™ (MALS verified)

  • Synonym
    Spike,S protein,Spike glycoprotein,S glycoprotein
  • Source
    Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His,Avitag (SPN-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 13 - Asn 1276 (Accession # Q0ZME7-1). Amino acid substitutions RRKRR 752-756 GGSGS and NL 1067-1068 PP are introduced to abolish the furin cleavage site and stabilize the trimeric prefusion state of the spike protein, respectively.
    Predicted N-terminus: Ala 13
  • Molecular Characterization
    Online(Ala 13 - Asn 1276) Q0ZME7-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 147.2 kDa. The protein migrates as 200-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His,Avitag (Cat. No. SPN-H82E3) SDS-PAGE gel

Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His,Avitag (Cat. No. SPN-H82E3) was more than 85% and the molecular weight of this protein is around 590-620 kDa verified by SEC-MALS.

  • Background
    Human coronavirus HKU1 (HCoV-HKU1) is one of seven known coronaviruses to infect humans. The virus is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by binding to the N-acetyl-9-O-acetylneuraminic acid receptor. It causes an upper respiratory disease with symptoms of the common cold, but can advance to pneumonia and bronchiolitis. The spike protein of HCoV-HKU1 is a trimer that contains two main subunits: the S1 subunit is responsible for receptor binding, while the S2 subunit mediates membrane fusion.
  • Research Progress of Spike protein
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $550.00

Price(USD) : $2200.00

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