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Your Position: Home > Fc Receptor Proteins > Fc gamma RIIIB / CD16b (NA2) > Human Fc gamma RIIIB / CD16b (NA2) Protein, His Tag (SPR & BLI verified)

Human Fc gamma RIIIB / CD16b (NA2) Protein, His Tag (SPR & BLI verified)

  • Synonym
    Fc gamma RIIIB,CD16b (NA2),FCGR3B,CD16B,FCG3B,FCGR3,FCG3,IGFR3
  • Source
    Human CD16b (NA2), His Tag (SPR & BLI verified) (CDB-H5222) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Ser 200 (Accession # O75015-1). The NA1 form of the CD16b differ with the NA2 form of CD16b in AA36, 65, 82, and 106. The NA1 form carries R36, N65, D82, and V106, while the NA2 form carries S36, S65, N82, and I106.
    Predicted N-terminus: Gly 17
  • Molecular Characterization
    Online(Gly 17 - Ser 200) O75015-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 22.7 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD16b (NA2), His Tag (SPR & BLI verified) (Cat. No. CDB-H5222) SDS-PAGE gel

Human CD16b (NA2), His Tag (SPR & BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Human CD16b (NA2), His Tag (SPR & BLI verified) (Cat. No. CDB-H5222) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 2.88 μM as determined in a SPR assay (Biacore T200) (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human CD16b (NA2), His Tag (SPR & BLI verified) (Cat. No. CDB-H5222) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 7.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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