>Fc Receptor Proteins
>Fc gamma RIIIB / CD16b (NA2)
>Human Fc gamma RIIIB / CD16b (NA2) Protein, His Tag (SPR & BLI verified)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.7 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human CD16b (NA2), His Tag (SPR & BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human CD16b (NA2), His Tag (SPR & BLI verified) (Cat. No. CDB-H5222) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 2.88 μM as determined in a SPR assay (Biacore T200) (QC tested).
Loaded Human CD16b (NA2), His Tag (SPR & BLI verified) (Cat. No. CDB-H5222) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 7.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Simonov V, et al
Journal: Biologicals 43480
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