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Your Position: Home > Immune Checkpoint Proteins > PD-1 > Human PD-1 /PDCD1 Protein

Human PD-1 / PDCD1 Protein (HPLC-verified)

  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    Human PD-1, His Tag (PD1-H5221) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_005009.2).
    N-terminal Sequence Analysis: Leu 25
  • Molecular Characterization
    Online(Leu 25 - Gln 167) NP_005009.2

    This protein carries a polyhistidine tag at the C-terminus, and has a calculated MW of 16.8 kDa. The N-terminus Sequence Analysis is Leu 25. The reducing (R) protein migrates as 31-44 kDa in SDS-PAGE  due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human PD-1, His Tag (Cat. No. PD1-H5221) SDS-PAGE gel

Human PD-1, His Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Human PD-1, His Tag (Cat. No. ) HPLC images

The purity of Human PD-1, His Tag (HPLC-verified)(Cat.# PD1-H5221) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
Human PD-1, His TagHuman PD-1, His Tag (Cat. No. PD1-H5221) ELISA bioactivity

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 0.2μg/mL (100 μL/well) can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with a linear range of 0.31-1.25 μg/mL (QC tested).

Human PD-1, His TagHuman PD-1, His Tag (Cat. No. PD1-H5221) ELISA bioactivity

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 0.2 μg/mL ( 100 μl/well ) can bind Human PD-L2, Fc Tag (Cat. No. PD2-H5251) with a linear range of 0.16-2.5 μg/mL (QC tested).

Human PD-1, His TagHuman PD-1, His Tag (Cat. No. PD1-H5221) ELISA bioactivity

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 2 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5) with a linear range of 10-156 ng/mL (Routinely tested).

Human PD-1, His TagHuman PD-1, His Tag (Cat. No. PD1-H5221) ELISA bioactivity

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Opdivo(Nivolumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-1, His Tag (Cat. No. PD1-H5221) with an affinity constant of 4.94 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

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